| Label | Region | Length (bp) | Pi | Alignment reliability | MarkerSeek score | Primer available | Action |
|---|---|---|---|---|---|---|---|
| trnC-GCA-rpoB | LSC | 1138 | 0.0035 | 1.00 | 60.8 | yes | View details |
| rpoC2-rps2 | LSC | 291 | 0.0069 | 1.00 | 42.9 | yes | View details |
| atpI | LSC | 744 | 0.0040 | 1.00 | 46.3 | yes | View details |
| rbcL-psaI | LSC | 1586 | 0.0090 | 0.70 | 61.2 | yes | View details |
| ndhF | SSC | 2220 | 0.0018 | 1.00 | 43.9 | yes | View details |
| ndhF-rpl32 | SSC | 906 | 0.0056 | 0.98 | 63.8 | yes | View details |
| rpl32-trnL-UAG | SSC | 647 | 0.0108 | 1.00 | 65.0 | yes | View details |
| atpF-atpA | LSC | 91 | 0.0220 | 1.00 | 65.1 | yes | View details |
| rps3 | LSC | 720 | 0.0028 | 1.00 | 60.3 | yes | View details |
| trnG-UCC-trnT-GGU | LSC | 1165 | 0.0017 | 1.00 | 60.1 | yes | View details |
| psbA | LSC | 1062 | 0.0019 | 1.00 | 59.8 | yes | View details |
| atpB-rbcL | LSC | 794 | 0.0013 | 1.00 | 59.7 | yes | View details |
| ndhH | SSC | 1182 | 0.0017 | 1.00 | 59.7 | yes | View details |
| rps16 | LSC | 1085 | 0.0009 | 1.00 | 59.6 | yes | View details |
| psaA-ycf3 | LSC | 642 | 0.0016 | 1.00 | 59.6 | yes | View details |
| Candidate markers include all hotspot labels marked in the diversity plot, listed in genomic order, plus the top 10 remaining regions by MarkerSeek score. Primer design is attempted for every listed region. Primer available: yes means at least one valid primer pair was found; no means no valid pair passed screening; NA means primer design was skipped. | |||||||
Species
2
Genome length
135–136 kb
Candidate markers
265
Primer pairs
85
Genome-wide nucleotide diversity
Candidate markers
7 hotspot labels from the diversity plot in genomic order, plus the top 8 remaining regions by MarkerSeek score (out of 265 candidates).
Primer pairs
Showing the top 30 of 85 primer pairs (ranked by primer score).
| Primer ID | Label | Forward | Reverse | Amplicon (bp) | Cross-species rate | Score |
|---|---|---|---|---|---|---|
| psbA_p1 | psbA | ACCCAATATCTTGCTGAAACA |
TATGTGTGCTTGGGAGTCCT |
1192 | 1.000 | 49.1 |
| psbA_p2 | psbA | TGCTGAAACAAGATATTGGGT |
TATGTGTGCTTGGGAGTCCT |
1181 | 1.000 | 49.1 |
| psbA_p3 | psbA | ACCCAATATCTTGCTGAAACAAG |
TATGTGTGCTTGGGAGTCCT |
1192 | 1.000 | 48.2 |
| psbA_p4 | psbA | ACCCAATATCTTGCTGAAACAA |
TATGTGTGCTTGGGAGTCCT |
1192 | 1.000 | 47.3 |
| psbA_p5 | psbA | TTGCTGAAACAAGATATTGGGT |
TATGTGTGCTTGGGAGTCCT |
1182 | 1.000 | 47.3 |
| trnK-UUU_p1 | trnK-UUU | AGGACTCCCAAGCACACATA |
GTAATCGGGGTCGCTCTTTT |
2978–2981 | 1.000 | 77.3 |
| trnK-UUU_p2 | trnK-UUU | AAAACACCGAACCATCCGAT |
CGTGCGGTGTAATTCCATTG |
2982–2985 | 1.000 | 77.3 |
| trnK-UUU_p3 | trnK-UUU | AGGACTCCCAAGCACACATA |
GCAGTTTGCCATCGTAATCG |
2991–2994 | 1.000 | 76.7 |
| trnK-UUU_p4 | trnK-UUU | GCTTGTACTTTCGCGTCTCT |
CGTGCGGTGTAATTCCATTG |
2911–2914 | 1.000 | 76.4 |
| trnK-UUU_p5 | trnK-UUU | CACCGAACCATCCGATGTAA |
CGTGCGGTGTAATTCCATTG |
2978–2981 | 1.000 | 76.4 |
| rps16_p1 | rps16 | GATTGGCGCGACATAAATCC |
ACCACTTTGCAATGAAAAGAGG |
1232–1235 | 1.000 | 59.2 |
| rps16_p2 | rps16 | GGATTGGCGCGACATAAATC |
ACCACTTTGCAATGAAAAGAGG |
1233–1236 | 1.000 | 59.2 |
| rps16_p3 | rps16 | ATTGGCGCGACATAAATCCA |
ACCACTTTGCAATGAAAAGAGG |
1231–1234 | 1.000 | 59.1 |
| rps16_p4 | rps16 | TGGATTGGCGCGACATAAAT |
ACCACTTTGCAATGAAAAGAGG |
1234–1237 | 1.000 | 59.1 |
| rps16_p5 | rps16 | TGGCGCGACATAAATCCAAA |
ACCACTTTGCAATGAAAAGAGG |
1229–1232 | 1.000 | 57.3 |
| trnG-UCC-trnT-GGU_p1 | trnG-UCC-trnT-GGU | ACGAATCACACTTTTACCACT |
CGTCTTAGCACGGCATTACT |
1306–1308 | 1.000 | 51.5 |
| trnG-UCC-trnT-GGU_p2 | trnG-UCC-trnT-GGU | ACGAATCACACTTTTACCACT |
CTTACGTCTTAGCACGGCAT |
1310–1312 | 1.000 | 51.5 |
| trnG-UCC-trnT-GGU_p3 | trnG-UCC-trnT-GGU | AGAACGAATCACACTTTTACCAC |
CGTCTTAGCACGGCATTACT |
1309–1311 | 1.000 | 50.4 |
| trnG-UCC-trnT-GGU_p4 | trnG-UCC-trnT-GGU | GAACGAATCACACTTTTACCACT |
CGTCTTAGCACGGCATTACT |
1308–1310 | 1.000 | 50.4 |
| trnG-UCC-trnT-GGU_p5 | trnG-UCC-trnT-GGU | GAACGAATCACACTTTTACCACT |
CTTACGTCTTAGCACGGCAT |
1312–1314 | 1.000 | 50.4 |
| trnC-GCA-rpoB_p1 | trnC-GCA-rpoB | AAAGGATTTGCAGTCCCCTG |
ATTCCCTCGTTTCCATTCCG |
1207 | 1.000 | 79.4 |
| trnC-GCA-rpoB_p2 | trnC-GCA-rpoB | AAAGGATTTGCAGTCCCCTG |
TGTGGACATTCCCTCGTTTC |
1214 | 1.000 | 78.3 |
| trnC-GCA-rpoB_p3 | trnC-GCA-rpoB | AAAGGATTTGCAGTCCCCTG |
TGTCAAGTTCTTCTGCCAGG |
1290 | 1.000 | 77.6 |
| trnC-GCA-rpoB_p4 | trnC-GCA-rpoB | AAAGGATTTGCAGTCCCCTG |
CCGGGTATTGTGGACATTCC |
1222 | 1.000 | 77.1 |
| trnC-GCA-rpoB_p5 | trnC-GCA-rpoB | AAAGGATTTGCAGTCCCCTG |
CGGGTATTGTGGACATTCCC |
1221 | 1.000 | 77.1 |
| rpoC2-rps2_p1 | rpoC2-rps2 | ATGAGACATCAGAACCCCCA |
TTTTCCCTGACTTGCTGCAT |
542 | 1.000 | 80.4 |
| rpoC2-rps2_p2 | rpoC2-rps2 | TGAGACATCAGAACCCCCAT |
TTTTCCCTGACTTGCTGCAT |
541 | 1.000 | 80.4 |
| rpoC2-rps2_p3 | rpoC2-rps2 | ATGAGACATCAGAACCCCCA |
GAGCCCTTATTGCAGCTGAT |
609 | 1.000 | 80.1 |
| rpoC2-rps2_p4 | rpoC2-rps2 | TGAGACATCAGAACCCCCAT |
GAGCCCTTATTGCAGCTGAT |
608 | 1.000 | 80.1 |
| rpoC2-rps2_p5 | rpoC2-rps2 | ACGCAAGGTCTTCCAAAAGT |
TTTTCCCTGACTTGCTGCAT |
1245 | 1.000 | 79.9 |
Result downloads
Reference species (2)
One GenBank record per species. Use the NCBI button to open the Nucleotide entry for the listed accession.
| Species | Accession | Length (bp) | Link |
|---|---|---|---|
| Puccinellia distans | NC_054281.1 | 135647 | View on NCBI ↗ |
| Puccinellia nuttalliana | NC_027485.1 | 135353 | View on NCBI ↗ |