Hotspot

ndhF-rpl32

SSC · 103799-104704 · 906 bp

Back to Puccinellia

Pi 0.00562

MarkerSeek score 63.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 906
variable_sites: 5
pi: 0.00562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGACATTGAAGTACGCTTTT	55.0	35.0	58.1	53.5	972-989 bp	1.0	44.6
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGCTTT	55.0	35.0	58.1	53.5	973-990 bp	1.0	44.6
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGCTT	55.0	35.0	58.1	53.5	974-991 bp	1.0	44.6
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGCT	55.0	35.0	58.1	53.5	975-992 bp	1.0	44.6
ndhF-rpl32_p5	GGCCAAACCCCATTGACATA	TGACATTGAAGTACGCTTTT	50.0	35.0	58.1	53.5	998-1015 bp	1.0	44.3