Hotspot

rps3

LSC · 78752-79471 · 720 bp

Back to Puccinellia

Pi 0.00278

MarkerSeek score 60.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 2
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCACTGGACAGAATGCAAAGA	TCCATCTAAGATTTCAATTCCCAGT	42.9	36.0	57.5	58.0	800 bp	1.0	40.2
rps3_p2	TCACTGGACAGAATGCAAAGA	CCATCTAAGATTTCAATTCCCAGT	42.9	37.5	57.5	56.7	799 bp	1.0	40.2
rps3_p3	TCACTGGACAGAATGCAAAGA	TCCATCTAAGATTTCAATTCCCAG	42.9	37.5	57.5	56.5	800 bp	1.0	40.2
rps3_p4	ATCACTGGACAGAATGCAAAGA	TCCATCTAAGATTTCAATTCCCAGT	40.9	36.0	58.0	58.0	801 bp	1.0	40.2
rps3_p5	TCACTGGACAGAATGCAAAGAT	TCCATCTAAGATTTCAATTCCCAGT	40.9	36.0	58.0	58.0	800 bp	1.0	40.2