Hotspot

rpoC2-rps2

LSC · 28987-29277 · 291 bp

Back to Puccinellia

Pi 0.00687

MarkerSeek score 42.90

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 2
pi: 0.00687
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	ATGAGACATCAGAACCCCCA	TTTTCCCTGACTTGCTGCAT	50.0	45.0	58.0	58.0	542 bp	1.0	80.4
rpoC2-rps2_p2	TGAGACATCAGAACCCCCAT	TTTTCCCTGACTTGCTGCAT	50.0	45.0	58.0	58.0	541 bp	1.0	80.4
rpoC2-rps2_p3	ATGAGACATCAGAACCCCCA	GAGCCCTTATTGCAGCTGAT	50.0	50.0	58.0	58.0	609 bp	1.0	80.1
rpoC2-rps2_p4	TGAGACATCAGAACCCCCAT	GAGCCCTTATTGCAGCTGAT	50.0	50.0	58.0	58.0	608 bp	1.0	80.1
rpoC2-rps2_p5	ACGCAAGGTCTTCCAAAAGT	TTTTCCCTGACTTGCTGCAT	45.0	45.0	57.9	58.0	1245 bp	1.0	79.9