Hotspot

atpB-rbcL

LSC · 53139-53932 · 794 bp

Back to Puccinellia

Pi 0.00126
MarkerSeek score 59.70
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
794
variable_sites
1
pi
0.00126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AAACCCCAGGACGAGAAGTA CCTTGGTTTCATACTCCGGG 50.0 55.0 58.0 58.0 926-928 bp 1.0 79.8
atpB-rbcL_p2 AAACCCCAGGACGAGAAGTA CTTGGTTTCATACTCCGGGG 50.0 55.0 58.0 58.0 925-927 bp 1.0 79.8
atpB-rbcL_p3 GGGAAAAGTGACATCCAGCA CCTTGGTTTCATACTCCGGG 50.0 55.0 58.1 58.0 997-999 bp 1.0 79.2
atpB-rbcL_p4 GGGAAAAGTGACATCCAGCA CTTGGTTTCATACTCCGGGG 50.0 55.0 58.1 58.0 996-998 bp 1.0 79.2
atpB-rbcL_p5 GGGGGAAAAGTGACATCCAG CTTGGTTTCATACTCCGGGG 55.0 55.0 58.2 58.0 998-1000 bp 1.0 78.6