Hotspot

atpF-atpA

LSC · 33636-33726 · 91 bp

Back to Puccinellia

Pi 0.022

MarkerSeek score 65.10

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 2
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	CCGTACAAGGAGCTCTAGGA	CATCCCCCACTTGAACTACG	55.0	55.0	57.9	58.0	313 bp	1.0	79.9
atpF-atpA_p2	CCGTACAAGGAGCTCTAGGA	TTGTTCAATACGTTCGCGGA	55.0	45.0	57.9	58.2	258 bp	1.0	78.4
atpF-atpA_p3	ATTTCCGTACGATTCGTGCT	CATCCCCCACTTGAACTACG	45.0	55.0	57.7	58.0	262 bp	1.0	78.1
atpF-atpA_p4	CATTTCCGTACGATTCGTGC	CATCCCCCACTTGAACTACG	50.0	55.0	57.7	58.0	263 bp	1.0	77.6
atpF-atpA_p5	CCGTACAAGGAGCTCTAGGA	TTCAATACGTTCGCGGAGAA	55.0	45.0	57.9	57.6	255 bp	1.0	77.4