Hotspot

rpl32-trnL-UAG

SSC · 104885-105531 · 647 bp

Back to Puccinellia

Pi 0.0108

MarkerSeek score 65.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 647
variable_sites: 7
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CATTTTCCAGCGGCAATGAG	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.1	58.0	778-779 bp	1.0	80.0
rpl32-trnL-UAG_p2	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	746-747 bp	1.0	79.7
rpl32-trnL-UAG_p3	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	745-746 bp	1.0	79.7
rpl32-trnL-UAG_p4	CATTTTCCAGCGGCAATGAG	GGATTCGAACCGAGATGCTT	50.0	50.0	58.1	58.1	781-782 bp	1.0	79.6
rpl32-trnL-UAG_p5	AGGGTTTTTCTGGGCAACAA	GGATTCGAACCGAGATGCTT	45.0	50.0	58.1	58.1	748-749 bp	1.0	79.3