Hotspot

rbcL-psaI

LSC · 55361-56946 · 1586 bp

Back to Puccinellia

Pi 0.00901

MarkerSeek score 61.20

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1586
variable_sites: 10
pi: 0.00901
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AGTTCGAGCCGGTAGATACT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.7	58.0	1259-1682 bp	1.0	81.6
rbcL-psaI_p2	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1273-1696 bp	1.0	81.5
rbcL-psaI_p3	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1278-1701 bp	1.0	81.2
rbcL-psaI_p4	AGTTCGAGCCGGTAGATACT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.7	57.9	1265-1688 bp	1.0	81.0
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.6	57.9	1279-1702 bp	1.0	80.9