Hotspot

psbA

LSC · 87-1148 · 1062 bp

Back to Puccinellia

Pi 0.00188

MarkerSeek score 59.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACCCAATATCTTGCTGAAACA	TATGTGTGCTTGGGAGTCCT	38.1	50.0	55.5	58.3	1192 bp	1.0	49.1
psbA_p2	TGCTGAAACAAGATATTGGGT	TATGTGTGCTTGGGAGTCCT	38.1	50.0	55.5	58.3	1181 bp	1.0	49.1
psbA_p3	ACCCAATATCTTGCTGAAACAAG	TATGTGTGCTTGGGAGTCCT	39.1	50.0	57.4	58.3	1192 bp	1.0	48.2
psbA_p4	ACCCAATATCTTGCTGAAACAA	TATGTGTGCTTGGGAGTCCT	36.4	50.0	56.2	58.3	1192 bp	1.0	47.3
psbA_p5	TTGCTGAAACAAGATATTGGGT	TATGTGTGCTTGGGAGTCCT	36.4	50.0	56.2	58.3	1182 bp	1.0	47.3