Hotspot

trnG-UCC-trnT-GGU

LSC · 13618-14782 · 1165 bp

Back to Puccinellia

Pi 0.00172

MarkerSeek score 60.10

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 2
pi: 0.00172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CGTCTTAGCACGGCATTACT	38.1	50.0	55.5	58.1	1306-1308 bp	1.0	51.5
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	CTTACGTCTTAGCACGGCAT	38.1	50.0	55.5	58.1	1310-1312 bp	1.0	51.5
trnG-UCC-trnT-GGU_p3	AGAACGAATCACACTTTTACCAC	CGTCTTAGCACGGCATTACT	39.1	50.0	57.3	58.1	1309-1311 bp	1.0	50.4
trnG-UCC-trnT-GGU_p4	GAACGAATCACACTTTTACCACT	CGTCTTAGCACGGCATTACT	39.1	50.0	57.3	58.1	1308-1310 bp	1.0	50.4
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	CTTACGTCTTAGCACGGCAT	39.1	50.0	57.3	58.1	1312-1314 bp	1.0	50.4