Hotspot

ycf1-rps15

Genome · 113507-114078 · 572 bp

Back to Glycyrrhiza

Pi 0.0132
MarkerSeek score 68.90
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
Genome
length_bp
572
variable_sites
41
pi
0.0132
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 ACCCATAATGGAAGCCGTTT TCAACTGATCCGGGGTTTTC 45.0 50.0 57.5 57.8 80-790 bp 1.0 83.2
ycf1-rps15_p2 ACCCATAATGGAAGCCGTTT CAACTGATCCGGGGTTTTCT 45.0 50.0 57.5 57.8 80-789 bp 1.0 83.2
ycf1-rps15_p3 ACCCATAATGGAAGCCGTTT ACTGATCCGGGGTTTTCTTC 45.0 50.0 57.5 57.5 80-787 bp 1.0 80.8
ycf1-rps15_p4 ACCCATAATGGAAGCCGTTT TTCAACTGATCCGGGGTTTT 45.0 45.0 57.5 57.3 80-791 bp 1.0 79.4
ycf1-rps15_p5 ACCCATAATGGAAGCCGTTT CTGATCCGGGGTTTTCTTCT 45.0 50.0 57.5 57.2 80-786 bp 1.0 78.5