Hotspot

rps8-rpl14

Genome · 80664-81050 · 387 bp

Back to Glycyrrhiza

Pi 0.0118

MarkerSeek score 60.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: Genome
length_bp: 387
variable_sites: 31
pi: 0.0118
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CAGCGATGGTATCTTTACCCA	GTTTCATTAGCACCCGAGGT	47.6	50.0	57.5	57.9	436-513 bp	1.0	76.6
rps8-rpl14_p2	AGCGATGGTATCTTTACCCAC	GTTTCATTAGCACCCGAGGT	47.6	50.0	57.5	57.9	435-512 bp	1.0	76.6
rps8-rpl14_p3	CAGCGATGGTATCTTTACCCA	CCCGGGAATTGAGACAGTTC	47.6	55.0	57.5	58.3	471-548 bp	1.0	75.1
rps8-rpl14_p4	AGCGATGGTATCTTTACCCAC	CCCGGGAATTGAGACAGTTC	47.6	55.0	57.5	58.3	470-547 bp	1.0	75.1
rps8-rpl14_p5	TCTATTCCTGTCAGCATTGCA	GTTTCATTAGCACCCGAGGT	42.9	50.0	57.4	57.9	474-551 bp	1.0	75.1