Hotspot

petN-trnC-GCA

Genome · 33925-34993 · 1069 bp

Back to Glycyrrhiza

Pi 0.0102

MarkerSeek score 62.40

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1069
variable_sites: 60
pi: 0.0102
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTTGTATCGTTTTGGCGGC	45.0	45.0	58.0	57.9	1185-1235 bp	1.0	87.6
petN-trnC-GCA_p2	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	1129-1179 bp	1.0	87.5
petN-trnC-GCA_p3	CAGCCCAAGCAAGACTTACT	TTTTGTATCGTTTTGGCGGC	50.0	45.0	57.8	57.9	1179-1229 bp	1.0	86.1
petN-trnC-GCA_p4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	1123-1173 bp	1.0	86.0
petN-trnC-GCA_p5	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	1147-1197 bp	1.0	85.3