Hotspot

psbM-petN

Genome · 33306-33834 · 529 bp

Back to Glycyrrhiza

Pi 0.00928

MarkerSeek score 60.90

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 529
variable_sites: 28
pi: 0.00928
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTCTTCATTCT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.0	58.0	568-685 bp	1.0	71.5
psbM-petN_p2	TGCTACTGCACTCTTCATTCT	AGTAAGTCTTGCTTGGGCTG	42.9	50.0	57.0	57.8	574-691 bp	1.0	70.0
psbM-petN_p3	TGCTACTGCACTCTTCATTCT	ATGGGGAAGAAGTGGACTCT	42.9	50.0	57.0	57.6	514-631 bp	1.0	69.0
psbM-petN_p4	TGCTACTGCACTCTTCATTC	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	55.5	58.0	568-685 bp	1.0	67.1
psbM-petN_p5	TGCTACTGCACTCTTCATTC	AGTAAGTCTTGCTTGGGCTG	45.0	50.0	55.5	57.8	574-691 bp	1.0	65.6