Hotspot

psbD-trnT-GGU

Genome · 29648-31130 · 1483 bp

Back to Glycyrrhiza

Pi 0.00664
MarkerSeek score 62.00
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
Genome
length_bp
1483
variable_sites
63
pi
0.00664
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 GAGCAATAGACCGGACCAAC CAGTGTGGTAGAGTAACGCC 55.0 55.0 58.3 58.0 1628-1703 bp 1.0 83.8
psbD-trnT-GGU_p2 GAGCAATAGACCGGACCAAC GTGGTAGAGTAACGCCATGG 55.0 55.0 58.3 58.1 1624-1699 bp 1.0 83.3
psbD-trnT-GGU_p3 AAGAGCAATAGACCGGACCA CAGTGTGGTAGAGTAACGCC 50.0 55.0 58.4 58.0 1630-1705 bp 1.0 83.1
psbD-trnT-GGU_p4 AGAGCAATAGACCGGACCAA CAGTGTGGTAGAGTAACGCC 50.0 55.0 58.4 58.0 1629-1704 bp 1.0 83.1
psbD-trnT-GGU_p5 AGAGCAATAGACCGGACCAA GTGGTAGAGTAACGCCATGG 50.0 55.0 58.4 58.1 1625-1700 bp 1.0 82.7