Hotspot

ycf3-psaA

Genome · 19538-20429 · 892 bp

Back to Glycyrrhiza

Pi 0.00728

MarkerSeek score 63.40

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: Genome
length_bp: 892
variable_sites: 35
pi: 0.00728
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	985-1083 bp	1.0	85.7
ycf3-psaA_p2	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.0	57.9	984-1082 bp	1.0	85.7
ycf3-psaA_p3	AGCTATAGCCCTTACCCCTG	TGCCCATTCCTCGAAAGAAG	55.0	50.0	58.0	57.9	991-1089 bp	1.0	85.5
ycf3-psaA_p4	AGCTATAGCCCTTACCCCTG	GCCCATTCCTCGAAAGAAGT	55.0	50.0	58.0	57.9	990-1088 bp	1.0	85.5
ycf3-psaA_p5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.1	57.9	949-1047 bp	1.0	85.1