Pi
0.0148
MarkerSeek score
77.20
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 1111-1766 bp | 1.0 | 89.2 |
| trnL-UAA-trnT-UGU_p2 | ACCAATTTCGCCATATCCCC | GAGTCGAACCGATGACCATC | 50.0 | 55.0 | 58.0 | 58.2 | 1142-1797 bp | 1.0 | 88.9 |
| trnL-UAA-trnT-UGU_p3 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 1119-1774 bp | 1.0 | 88.6 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GAGTCGAACCGATGACCATC | 50.0 | 55.0 | 58.1 | 58.2 | 1150-1805 bp | 1.0 | 88.3 |
| trnL-UAA-trnT-UGU_p5 | AGTCCGTAGCGTCTACCAAT | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.2 | 57.9 | 1125-1780 bp | 1.0 | 87.3 |