Hotspot

trnV-UAC-ndhC

Genome · 10570-11006 · 437 bp

Back to Glycyrrhiza

Pi 0.0169

MarkerSeek score 63.90

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 437
variable_sites: 31
pi: 0.0169
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	TTCTGGCCCTTTGCTAATCG	50.0	50.0	57.8	58.2	587-653 bp	0.961538	81.7
trnV-UAC-ndhC_p2	GAAGGTCTACGGTTCGAGTC	TTCTGGCCCTTTGCTAATCG	55.0	50.0	57.5	58.2	586-652 bp	0.961538	79.0
trnV-UAC-ndhC_p3	GTTCGAGTCCGTATAGCCCT	TTCTGGCCCTTTGCTAATCG	55.0	50.0	58.7	58.2	575-641 bp	0.961538	78.0
trnV-UAC-ndhC_p4	AAGGTCTACGGTTCGAGTCC	TTCTGGCCCTTTGCTAATCG	55.0	50.0	58.8	58.2	585-651 bp	0.961538	76.7
trnV-UAC-ndhC_p5	GAGAAGGTCTACGGTTCGAG	TTCTGGCCCTTTGCTAATCG	55.0	50.0	57.2	58.2	588-654 bp	0.961538	76.7