Hotspot

rps16

LSC · 4484-5572 · 1089 bp

Pi 0.0352

MarkerSeek score 71.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1089
variable_sites: 566
pi: 0.0352
unique_haplotype_count: 178
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCTTTCAGGATCAGTCGTGG	CCATAGGATAGAGGGGCGAA	55.0	55.0	58.0	58.1	1351-2048 bp	0.863436	85.9
rps16_p2	TCAGGATCAGTCGTGGTCTT	CCATAGGATAGAGGGGCGAA	50.0	55.0	58.1	58.1	1347-2044 bp	0.863436	85.4
rps16_p3	TTCAGGATCAGTCGTGGTCT	CCATAGGATAGAGGGGCGAA	50.0	55.0	58.1	58.1	1348-2045 bp	0.863436	85.4
rps16_p4	CCTTTCAGGATCAGTCGTGG	AGCAACAGCAGCTCTTTGAT	55.0	45.0	58.0	58.1	2151-2540 bp	0.444934	68.9
rps16_p5	CCTTTCAGGATCAGTCGTGG	ATTTCAGCAACAGCAGCTCT	55.0	45.0	58.0	58.1	2156-2545 bp	0.400881	67.2