Hotspot

ndhF

SSC · 109685-111901 · 2217 bp

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Pi 0.0256
MarkerSeek score 67.70
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2217
variable_sites
694
pi
0.0256
unique_haplotype_count
192
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGAACCGCCCATTGAGATAT CCCGTAGAAACTGACTTTGCT 50.0 47.6 57.4 58.2 2866-3000 bp 0.255507 46.0
ndhF_p2 GGAACCGCCCATTGAGATAT CCCGTAGAAACTGACTTTGC 50.0 50.0 57.4 56.7 2866-3000 bp 0.255507 45.8
ndhF_p3 GGAACCGCCCATTGAGATAT TCCCGTAGAAACTGACTTTGC 50.0 47.6 57.4 58.2 2867-2999 bp 0.246696 45.5
ndhF_p4 TGGAACCGCCCATTGAGATA CCCGTAGAAACTGACTTTGCT 50.0 47.6 58.8 58.2 2867-2999 bp 0.246696 43.8
ndhF_p5 TGGAACCGCCCATTGAGATA TCCCGTAGAAACTGACTTTGC 50.0 47.6 58.8 58.2 2868-3000 bp 0.246696 43.7