Hotspot

ccsA-ndhD

SSC · 114270-114487 · 218 bp

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Pi 0.0595
MarkerSeek score 71.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
218
variable_sites
106
pi
0.0595
unique_haplotype_count
158
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGCGGCTCTTTTATGTGGTT TTCTCGATGGCTTCTCTTGC 45.0 50.0 58.1 58.0 1075-1135 bp 0.969163 89.9
ccsA-ndhD_p2 ATGCGGCTCTTTTATGTGGT TTCTCGATGGCTTCTCTTGC 45.0 50.0 57.9 58.0 1076-1136 bp 0.969163 89.6
ccsA-ndhD_p3 TGCGGCTCTTTTATGTGGTT GGATTCTGGACCACGAGAAC 45.0 55.0 58.1 58.0 851-911 bp 0.960352 89.6
ccsA-ndhD_p4 ATGCGGCTCTTTTATGTGGT GGATTCTGGACCACGAGAAC 45.0 55.0 57.9 58.0 852-912 bp 0.960352 89.3
ccsA-ndhD_p5 TGCGGCTCTTTTATGTGGTT CTCGATGGCTTCTCTTGCAT 45.0 50.0 58.1 58.0 1073-1133 bp 0.960352 89.3