Hotspot

rpl32-trnL_UAG

SSC · 112458-113157 · 700 bp

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Pi 0.0727
MarkerSeek score 81.40
Primer available yes
Label rpl32-trnL_UAG

Feature Metadata

label_name
rpl32-trnL_UAG
feature_type
igs
region
SSC
length_bp
700
variable_sites
451
pi
0.0727
unique_haplotype_count
200
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_UAG_p1 CAAACAAATAAGAAAATCTTGGAA TCCTAAGAGCAGCGTGTCTA 25.0 50.0 52.7 57.9 625-929 bp 0.911894 48.5
rpl32-trnL_UAG_p2 CAAACAAATAAGAAAATCTTGGAA AGCAGCGTGTCTACCAATTT 25.0 45.0 52.7 57.8 618-922 bp 0.911894 48.5
rpl32-trnL_UAG_p3 CAAACAAATAAGAAAATCTTGGAA CCTAAGAGCAGCGTGTCTAC 25.0 55.0 52.7 57.8 624-928 bp 0.911894 48.5
rpl32-trnL_UAG_p4 CAAACAAATAAGAAAATCTTGGAA CTAAGAGCAGCGTGTCTACC 25.0 55.0 52.7 57.8 623-927 bp 0.911894 48.5
rpl32-trnL_UAG_p5 CAAACAAATAAGAAAATCTTGGAA GAGCAGCGTGTCTACCAATT 25.0 50.0 52.7 58.3 619-923 bp 0.911894 48.5