Hotspot

petA-psbJ

LSC · 62317-63473 · 1157 bp

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Pi 0.0445
MarkerSeek score 77.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1157
variable_sites
523
pi
0.0445
unique_haplotype_count
188
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTTGAAAAGGTTCA GGGTTGGGTTCATCTCTGTA 31.8 50.0 54.6 56.5 930-1287 bp 1.0 52.0
petA-psbJ_p2 AAGAAACAGTTTGAAAAGGTTCA GGGTTGGGTTCATCTCTGTA 30.4 50.0 55.4 56.5 931-1288 bp 1.0 52.0
petA-psbJ_p3 AGAAACAGTTTGAAAAGGTTCA GGGTTGGGTTCATCTCTGTAG 31.8 52.4 54.6 57.7 930-1287 bp 0.995595 51.8
petA-psbJ_p4 AAGAAACAGTTTGAAAAGGTTCA GGGTTGGGTTCATCTCTGTAG 30.4 52.4 55.4 57.7 931-1288 bp 0.995595 51.8
petA-psbJ_p5 AAAGAAACAGTTTGAAAAGGTTCA GGGTTGGGTTCATCTCTGTAG 29.2 52.4 56.1 57.7 932-1289 bp 0.995595 51.8