Hotspot

ndhC-trnV_UAC

LSC · 50768-51212 · 445 bp

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Pi 0.0408
MarkerSeek score 75.30
Primer available yes
Label ndhC-trnV_UAC

Feature Metadata

label_name
ndhC-trnV_UAC
feature_type
igs
region
LSC
length_bp
445
variable_sites
203
pi
0.0408
unique_haplotype_count
176
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV_UAC_p1 GCCAAAATAGGAATAGCACCC AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.0 57.8 489-697 bp 0.969163 73.1
ndhC-trnV_UAC_p2 CCAAAATAGGAATAGCACCCGA AGAAGGTCTACGGTTCGAGT 45.5 50.0 58.2 57.8 488-696 bp 0.982379 72.2
ndhC-trnV_UAC_p3 CCAAAATAGGAATAGCACCCG AGAAGGTCTACGGTTCGAGT 47.6 50.0 56.7 57.8 488-696 bp 0.982379 71.6
ndhC-trnV_UAC_p4 GCCAAAATAGGAATAGCACCC GAAGGTCTACGGTTCGAGTC 47.6 55.0 57.0 57.5 488-696 bp 0.969163 70.4
ndhC-trnV_UAC_p5 GCCAAAATAGGAATAGCACCC GTTCGAGTCCGTATAGCCCT 47.6 55.0 57.0 58.7 477-685 bp 0.969163 69.2