Hotspot

trnT_GGU-psbD

LSC · 32232-33289 · 1058 bp

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Pi 0.0426
MarkerSeek score 76.00
Primer available yes
Label trnT_GGU-psbD

Feature Metadata

label_name
trnT_GGU-psbD
feature_type
igs
region
LSC
length_bp
1058
variable_sites
506
pi
0.0426
unique_haplotype_count
182
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT_GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ATTTTACCAAGAGCTATAGTCA 55.0 31.8 58.1 52.3 798-1632 bp 1.0 52.0
trnT_GGU-psbD_p2 GTGGTAGAGTAACGCCATGG TTTTACCAAGAGCTATAGTCAT 55.0 31.8 58.1 52.3 797-1631 bp 1.0 52.0
trnT_GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA ATTTTACCAAGAGCTATAGTCA 50.0 31.8 58.1 52.3 801-1635 bp 1.0 52.0
trnT_GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TTTTACCAAGAGCTATAGTCAT 50.0 31.8 58.1 52.3 800-1634 bp 1.0 52.0
trnT_GGU-psbD_p5 GTGGTAGAGTAACGCCATGG AATTTTACCAAGAGCTATAGTCA 55.0 30.4 58.1 53.2 799-1633 bp 1.0 52.0