Hotspot

rpoB-trnC_GCA

LSC · 26867-28117 · 1251 bp

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Pi 0.0358
MarkerSeek score 74.60
Primer available yes
Label rpoB-trnC_GCA

Feature Metadata

label_name
rpoB-trnC_GCA
feature_type
igs
region
LSC
length_bp
1251
variable_sites
545
pi
0.0358
unique_haplotype_count
196
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC_GCA_p1 TGTGGACATTCCCTCATTTCC GCCTTACCACTTGGCCATG 47.6 57.9 57.9 58.8 893-1344 bp 0.933921 66.2
rpoB-trnC_GCA_p2 GTGGACATTCCCTCATTTCCA GCCTTACCACTTGGCCATG 47.6 57.9 57.9 58.8 892-1343 bp 0.920705 65.7
rpoB-trnC_GCA_p3 CCTCATTTCCATTCCGAAGC CCTTACCACTTGGCCATGC 50.0 57.9 56.9 58.8 881-1332 bp 0.929515 65.6
rpoB-trnC_GCA_p4 TGTGGACATTCCCTCATTTCC CCTTACCACTTGGCCATGC 47.6 57.9 57.9 58.8 892-1343 bp 0.907489 65.2
rpoB-trnC_GCA_p5 GTGGACATTCCCTCATTTCCA CCTTACCACTTGGCCATGC 47.6 57.9 57.9 58.8 891-1342 bp 0.894273 64.6