Hotspot

atpH-atpI

LSC · 14168-14657 · 490 bp

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Pi 0.0246
MarkerSeek score 72.60
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
490
variable_sites
211
pi
0.0246
unique_haplotype_count
158
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT ATAGGGGAATCCATGGAGGG 45.0 55.0 58.0 57.9 351-603 bp 0.982379 90.5
atpH-atpI_p2 CGCAATACCTTCTACGGCTT ATAGGGGAATCCATGGAGGG 50.0 55.0 58.1 57.9 439-691 bp 0.973568 89.9
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA ATAGGGGAATCCATGGAGGG 45.0 55.0 58.2 57.9 352-604 bp 0.982379 89.3
atpH-atpI_p4 TACCTTGACCAACTCCAGGT ATAGGGGAATCCATGGAGGG 50.0 55.0 57.9 57.9 407-659 bp 0.969163 89.3
atpH-atpI_p5 GCAGTACCTTGACCAACTCC ATAGGGGAATCCATGGAGGG 55.0 55.0 58.2 57.9 411-663 bp 0.977974 89.1