Hotspot

trnG_UCC-trnR_UCU

LSC · 10244-10374 · 131 bp

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Pi 0.0541
MarkerSeek score 72.60
Primer available yes
Label trnG_UCC-trnR_UCU

Feature Metadata

label_name
trnG_UCC-trnR_UCU
feature_type
igs
region
LSC
length_bp
131
variable_sites
68
pi
0.0541
unique_haplotype_count
117
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG_UCC-trnR_UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 183-246 bp 1.0 75.3
trnG_UCC-trnR_UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 186-249 bp 1.0 75.1
trnG_UCC-trnR_UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 189-252 bp 1.0 74.7
trnG_UCC-trnR_UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 192-255 bp 1.0 74.5
trnG_UCC-trnR_UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 187-250 bp 1.0 72.9