Hotspot

trnK-UUU-rps16

LSC · 3920-4483 · 564 bp

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Pi 0.0486
MarkerSeek score 76.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
564
variable_sites
319
pi
0.0486
unique_haplotype_count
181
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG ATTGATGTTCGATCCCGACG 55.0 50.0 58.0 58.1 752-923 bp 0.938326 88.2
trnK-UUU-rps16_p2 TCAGGATCAGTCGTGGTCTT ATTGATGTTCGATCCCGACG 50.0 50.0 58.1 58.1 748-919 bp 0.938326 87.8
trnK-UUU-rps16_p3 TTCAGGATCAGTCGTGGTCT ATTGATGTTCGATCCCGACG 50.0 50.0 58.1 58.1 749-920 bp 0.938326 87.8
trnK-UUU-rps16_p4 ATCGCACTTAAAAGCCGAGT ATTGATGTTCGATCCCGACG 45.0 50.0 57.9 58.1 667-838 bp 0.938326 87.6
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG GGTGCTCAACCTACAGGAAC 55.0 55.0 58.0 58.2 665-809 bp 0.84141 84.0