Hotspot

rps7-trnL-CAA

IRa · 100009-100768 · 760 bp

Back to Zygophyllum

Pi 0.0705
MarkerSeek score 88.50
Primer available yes
Label rps7-trnL-CAA

Feature Metadata

label_name
rps7-trnL-CAA
feature_type
igs
region
IRa
length_bp
760
variable_sites
112
pi
0.0705
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
88.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-trnL-CAA_p1 ATGGCAGAGGCAAATAGAGC GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 578-1015 bp 1.0 91.8
rps7-trnL-CAA_p2 ATGGCAGAGGCAAATAGAGC CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 576-1013 bp 1.0 91.8
rps7-trnL-CAA_p3 GGCAGAGGCAAATAGAGCTT GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 576-1013 bp 1.0 91.5
rps7-trnL-CAA_p4 GGCAGAGGCAAATAGAGCTT CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 574-1011 bp 1.0 91.5
rps7-trnL-CAA_p5 ATGGCAGAGGCAAATAGAGC TACCATTTCACCACCAAGGC 50.0 50.0 58.0 58.1 566-1003 bp 1.0 91.2