Hotspot

trnV-GAC-rps12

SSC · 97119-98693 · 1575 bp

Back to Zygophyllum

Pi 0.0284
MarkerSeek score 70.20
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
SSC
length_bp
1575
variable_sites
87
pi
0.0284
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 AAGTGACACTCTACCGCTGA TCGTACTCTGGCAACTTTACG 50.0 47.6 58.4 58.1 1607-1660 bp 1.0 76.7
trnV-GAC-rps12_p2 TGACACTCTACCGCTGAGTT TCGTACTCTGGCAACTTTACG 50.0 47.6 58.4 58.1 1604-1657 bp 1.0 76.7
trnV-GAC-rps12_p3 TCAAAGTGACACTCTACCGC TCGTACTCTGGCAACTTTACG 50.0 47.6 57.6 58.1 1610-1663 bp 1.0 76.3
trnV-GAC-rps12_p4 AAAGTGACACTCTACCGCTG TCGTACTCTGGCAACTTTACG 50.0 47.6 57.6 58.1 1608-1661 bp 1.0 76.2
trnV-GAC-rps12_p5 CAAAGTGACACTCTACCGCT TCGTACTCTGGCAACTTTACG 50.0 47.6 57.6 58.1 1609-1662 bp 1.0 76.2