Hotspot

trnI-GAU-rrn16S

SSC · 95022-95366 · 345 bp

Back to Zygophyllum

Pi 0.038

MarkerSeek score 80.40

Primer available yes

Label trnI-GAU-rrn16S

Feature Metadata

label_name: trnI-GAU-rrn16S
feature_type: igs
region: SSC
length_bp: 345
variable_sites: 31
pi: 0.038
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16S_p1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	435-458 bp	1.0	82.8
trnI-GAU-rrn16S_p2	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	467-490 bp	1.0	82.5
trnI-GAU-rrn16S_p3	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	57.8	57.7	486-509 bp	1.0	82.2
trnI-GAU-rrn16S_p4	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	55.0	50.0	57.8	58.3	489-512 bp	1.0	82.1
trnI-GAU-rrn16S_p5	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	57.8	58.3	508-531 bp	1.0	81.9