Hotspot

rpl33-rps18

LSC · 63468-63877 · 410 bp

Back to Zygophyllum

Pi 0.0351

MarkerSeek score 80.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 28
pi: 0.0351
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAATCGACACAATACGCCGA	TAATCAATTCGATCCCCCGG	45.0	50.0	58.0	57.1	582-628 bp	1.0	80.9
rpl33-rps18_p2	AATCGACACAATACGCCGAA	TAATCAATTCGATCCCCCGG	45.0	50.0	58.0	57.1	581-627 bp	1.0	80.9
rpl33-rps18_p3	CGCCGAATCGATTGGAATTG	TAATCAATTCGATCCCCCGG	50.0	50.0	58.0	57.1	568-614 bp	1.0	80.8
rpl33-rps18_p4	AAATCGACACAATACGCCGA	GCAACCGCCTACGAAAAGAT	45.0	50.0	58.0	58.9	551-597 bp	1.0	80.6
rpl33-rps18_p5	AATCGACACAATACGCCGAA	GCAACCGCCTACGAAAAGAT	45.0	50.0	58.0	58.9	550-596 bp	1.0	80.6