Hotspot

trnT-UGU-trnL-UAA

LSC · 45592-46476 · 885 bp

Back to Zygophyllum

Pi 0.021

MarkerSeek score 71.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 40
pi: 0.021
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	923-953 bp	1.0	84.4
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	954-984 bp	1.0	84.1
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	917-947 bp	1.0	83.1
trnT-UGU-trnL-UAA_p4	AAAAGCCAGCTATCGGAGTC	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.7	58.2	969-999 bp	1.0	82.7
trnT-UGU-trnL-UAA_p5	GAAAAGCCAGCTATCGGAGT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.7	58.2	970-1000 bp	1.0	82.7