Hotspot

trnG-GCC-trnfM-CAU

LSC · 35434-35593 · 160 bp

Back to Zygophyllum

Pi 0.0355

MarkerSeek score 72.20

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 11
pi: 0.0355
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	225-244 bp	1.0	86.0
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	261-280 bp	1.0	85.7
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	223-242 bp	1.0	85.0
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	246-265 bp	1.0	84.3
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	245-264 bp	1.0	84.3