Hotspot

trnE-UUC-trnT-GGU

LSC · 29455-30114 · 660 bp

Back to Zygophyllum

Pi 0.0271

MarkerSeek score 76.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 40
pi: 0.0271
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	764-1145 bp	1.0	85.0
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	801-1182 bp	1.0	84.9
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	729-1110 bp	1.0	84.4
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	766-1147 bp	1.0	84.3
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	798-1179 bp	1.0	83.7