Hotspot

atpI-rps2

LSC · 14079-14254 · 176 bp

Back to Zygophyllum

Pi 0.05

MarkerSeek score 73.90

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 18
pi: 0.05
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GGAACCCACGGACCATATTC	ATTTCGATTCCGGCGAATGA	55.0	45.0	58.0	58.1	571-577 bp	1.0	82.5
atpI-rps2_p2	GGAACCCACGGACCATATTC	TTTCGATTCCGGCGAATGAT	55.0	45.0	58.0	58.1	570-576 bp	1.0	82.5
atpI-rps2_p3	GGAACCCACGGACCATATTC	AATTGTGATCCCGATCTCGC	55.0	50.0	58.0	58.1	595-601 bp	1.0	81.9
atpI-rps2_p4	CCCCTGACCAGTTAGAAACG	ATTTCGATTCCGGCGAATGA	55.0	45.0	57.9	58.1	621-627 bp	1.0	81.8
atpI-rps2_p5	CCCCTGACCAGTTAGAAACG	TTTCGATTCCGGCGAATGAT	55.0	45.0	57.9	58.1	620-626 bp	1.0	81.8