Hotspot

trnQ-UUG-psbK

LSC · 4878-5879 · 1002 bp

Back to Zygophyllum

Pi 0.0343

MarkerSeek score 71.20

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 63
pi: 0.0343
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TACTCTGGGACGGAAGGATT	CGAAAACTGACAGCAGCTTG	50.0	50.0	57.5	58.0	1106-1274 bp	1.0	83.8
trnQ-UUG-psbK_p2	TACTCTGGGACGGAAGGATT	GCACAGGAATGACTGGCATA	50.0	50.0	57.5	58.0	1060-1228 bp	1.0	83.8
trnQ-UUG-psbK_p3	TACTCTGGGACGGAAGGATT	TCGAAAACTGACAGCAGCTT	50.0	45.0	57.5	58.0	1107-1275 bp	1.0	83.7
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	CGAAAACTGACAGCAGCTTG	50.0	50.0	58.7	58.0	1068-1236 bp	1.0	82.9
trnQ-UUG-psbK_p5	TACTCTGGGACGGAAGGATT	ATCGAAAACTGACAGCAGCT	50.0	45.0	57.5	57.8	1108-1276 bp	1.0	82.6