Hotspot

ndhD-psaC

SSC · 126201-126319 · 119 bp

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Pi 0.0513
MarkerSeek score 76.60
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
119
variable_sites
6
pi
0.0513
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 CCGCGGATATTGGGAAAACT AAACAACTCGAAGCATGGGT 50.0 45.0 58.3 58.0 210-212 bp 1.0 80.3
ndhD-psaC_p2 AGAAGATTAAGGAACCCGCG AAACAACTCGAAGCATGGGT 50.0 45.0 57.7 58.0 225-227 bp 1.0 80.2
ndhD-psaC_p3 CCGCGGATATTGGGAAAACT GGCATGAAACAACTCGAAGC 50.0 50.0 58.3 58.0 216-218 bp 1.0 80.1
ndhD-psaC_p4 AGAAGATTAAGGAACCCGCG GGCATGAAACAACTCGAAGC 50.0 50.0 57.7 58.0 231-233 bp 1.0 80.0
ndhD-psaC_p5 TAAGGAACCCGCGGATATTG AAACAACTCGAAGCATGGGT 50.0 45.0 57.5 58.0 218-220 bp 1.0 78.5