Hotspot

trnL-UAG-rpl32

SSC · 120501-120951 · 451 bp

Back to Zygia

Pi 0.069
MarkerSeek score 77.20
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
451
variable_sites
29
pi
0.069
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AATTAGCTGGACTCAAAGAA 50.0 35.0 58.0 52.1 501-503 bp 1.0 48.6
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AATTAGCTGGACTCAAAGAA 50.0 35.0 58.0 52.1 502-504 bp 1.0 48.6
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA AATTAGCTGGACTCAAAGAA 50.0 35.0 57.9 52.1 511-513 bp 1.0 48.5
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC AATTAGCTGGACTCAAAGAA 55.0 35.0 57.8 52.1 510-512 bp 1.0 48.5
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC AATTAGCTGGACTCAAAGAA 55.0 35.0 57.8 52.1 509-511 bp 1.0 48.5