Hotspot

trnN-GUU-ccsA

IRb · 117008-119362 · 2355 bp

Back to Zygia

Pi 0.0399
MarkerSeek score 68.20
Primer available yes
Label trnN-GUU-ccsA

Feature Metadata

label_name
trnN-GUU-ccsA
feature_type
igs
region
IRb
length_bp
2355
variable_sites
84
pi
0.0399
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ccsA_p1 GCAAAAATGCCTTCCTCGTC AATGAGGCCTGGGGATCATA 50.0 50.0 58.0 57.9 2986 bp 0.5 58.8
trnN-GUU-ccsA_p2 GCAAAAATGCCTTCCTCGTC TAATGAGGCCTGGGGATCAT 50.0 50.0 58.0 57.9 2987 bp 0.5 58.8
trnN-GUU-ccsA_p3 GCAAAAATGCCTTCCTCGTC TGAGGCCTGGGGATCATATT 50.0 50.0 58.0 57.9 2984 bp 0.5 58.8
trnN-GUU-ccsA_p4 CGGGAGAATTGATGACTGCA AATGAGGCCTGGGGATCATA 50.0 50.0 58.0 57.9 2932 bp 0.5 58.7
trnN-GUU-ccsA_p5 CGGGAGAATTGATGACTGCA TGAGGCCTGGGGATCATATT 50.0 50.0 58.0 57.9 2930 bp 0.5 58.7