Hotspot

rps12-clpP

LSC · 76842-77139 · 298 bp

Back to Zygia

Pi 0.0917
MarkerSeek score 70.00
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
298
variable_sites
22
pi
0.0917
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 CCCTCTTAGTCCGTTCTTGC AGATGCCTGTACGGTTGTTC 55.0 50.0 58.0 57.9 1094-1124 bp 1.0 89.3
rps12-clpP_p2 GTGAAGATTAGCTGGGGAGC AGATGCCTGTACGGTTGTTC 55.0 50.0 58.0 57.9 1117-1147 bp 1.0 88.9
rps12-clpP_p3 CCCTCTTAGTCCGTTCTTGC TCCTGGAAGTGAGCGAAGTA 55.0 50.0 58.0 58.1 934 bp 0.5 59.3
rps12-clpP_p4 GTGAAGATTAGCTGGGGAGC TCCTGGAAGTGAGCGAAGTA 55.0 50.0 58.0 58.1 957 bp 0.5 59.0
rps12-clpP_p5 ACTTCTTAAACGGCGAGGTC TCCTGGAAGTGAGCGAAGTA 50.0 50.0 57.9 58.1 1126 bp 0.5 58.7