Hotspot

psbL-psbF

LSC · 70757-70778 · 22 bp

Back to Zygia

Pi 0.0455
MarkerSeek score 72.60
Primer available yes
Label psbL-psbF

Feature Metadata

label_name
psbL-psbF
feature_type
igs
region
LSC
length_bp
22
variable_sites
1
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-psbF_p1 GTTTGGGTTTGATTGTGTCGT AGTACGATGGTTGGCTGTTC 42.9 50.0 57.6 57.9 131 bp 1.0 68.4
psbL-psbF_p2 GTTTGGGTTTGATTGTGTCGT CAGTACGATGGTTGGCTGTT 42.9 50.0 57.6 58.2 132 bp 1.0 67.6
psbL-psbF_p3 GTTTGGGTTTGATTGTGTCGT GTACGATGGTTGGCTGTTCA 42.9 50.0 57.6 58.2 130 bp 1.0 67.6
psbL-psbF_p4 GTTTGGGTTTGATTGTGTCGT TACGATGGTTGGCTGTTCAC 42.9 50.0 57.6 58.2 129 bp 1.0 67.6
psbL-psbF_p5 CGTTTGGGTTTGATTGTGTCG AGTACGATGGTTGGCTGTTC 47.6 50.0 58.6 57.9 132 bp 1.0 66.9