Hotspot

ndhC-trnV-UAC

LSC · 55686-56616 · 931 bp

Back to Zygia

Pi 0.0325
MarkerSeek score 73.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
931
variable_sites
29
pi
0.0325
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTCGA AAAAGATGCACGTTGGGTCT 37.5 45.0 58.0 58.0 1107-1217 bp 1.0 52.5
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTCGA AGAAGGTCTACGGTTCGAGT 37.5 50.0 58.0 57.8 1036-1146 bp 1.0 51.4
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTCGA GATGCACGTTGGGTCTGTAA 37.5 50.0 58.0 58.2 1103-1213 bp 1.0 51.1
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTCGA ATGCACGTTGGGTCTGTAAA 37.5 45.0 58.0 57.7 1102-1212 bp 1.0 50.5
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTCG AAAAGATGCACGTTGGGTCT 39.1 45.0 56.7 58.0 1107-1217 bp 1.0 50.0