Pi
0.0726
MarkerSeek score
88.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1324-1662 bp | 1.0 | 83.7 |
| trnT-UGU-trnL-UAA_p2 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1332-1670 bp | 1.0 | 83.1 |
| trnT-UGU-trnL-UAA_p3 | AAATGCGACGCTCTAACCTC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.4 | 58.0 | 1296-1634 bp | 1.0 | 82.8 |
| trnT-UGU-trnL-UAA_p4 | AAATGCGACGCTCTAACCTC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.4 | 58.1 | 1304-1642 bp | 1.0 | 82.3 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | AGTCCGTAGCGTCTACCAAT | 50.0 | 50.0 | 57.8 | 58.2 | 1338-1676 bp | 1.0 | 81.7 |