Hotspot

trnR-UCU-atpA

LSC · 10975-11498 · 524 bp

Back to Zygia

Pi 0.0547
MarkerSeek score 72.60
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
524
variable_sites
15
pi
0.0547
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.52
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC CATTCACTCGGGAAGCAGAA 47.6 50.0 57.3 57.9 388-638 bp 1.0 72.0
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC AAGACATTCACTCGGGAAGC 47.6 50.0 57.3 57.9 392-642 bp 1.0 72.0
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC GGAAGCAGAAGCCCTTTTGA 47.6 50.0 57.3 58.4 378-628 bp 1.0 69.8
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC ATTCACTCGGGAAGCAGAAG 47.6 50.0 57.3 57.6 387-637 bp 1.0 69.7
trnR-UCU-atpA_p5 TGGATAGGACAGAGGTCTTCT CATTCACTCGGGAAGCAGAA 47.6 50.0 56.9 57.9 423-673 bp 1.0 69.0