Hotspot

trnS-GCU-trnG-UCC

LSC · 8997-9762 · 766 bp

Back to Zygia

Pi 0.147
MarkerSeek score 86.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
766
variable_sites
112
pi
0.147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 878-1271 bp 1.0 82.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 877-1270 bp 1.0 82.6
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.2 57.3 932-1325 bp 1.0 81.4
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 846-1239 bp 1.0 80.1
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 845-1238 bp 1.0 80.1