Hotspot

trnK-UUU-rps16

LSC · 4455-5514 · 1060 bp

Back to Zygia

Pi 0.0525
MarkerSeek score 74.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1060
variable_sites
55
pi
0.0525
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCTGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1295-1610 bp 1.0 83.6
trnK-UUU-rps16_p2 CCTTTCTGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1433-1748 bp 1.0 83.2
trnK-UUU-rps16_p3 CCTTTCTGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 1434-1749 bp 1.0 83.2
trnK-UUU-rps16_p4 CCTTTCTGGATCAGTCGTGG AAGCTAACGGATCGTGGAAC 55.0 50.0 58.0 58.0 1844-2160 bp 1.0 83.1
trnK-UUU-rps16_p5 CCTTTCTGGATCAGTCGTGG TGGGTATGTTGCTGCCATTT 55.0 45.0 58.0 58.1 1917-2233 bp 1.0 82.5