Hotspot

rpl16

LSC · 78494-79973 · 1480 bp

Pi 0.0023

MarkerSeek score 37.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1480
variable_sites: 10
pi: 0.0023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTCTCAGTTCCTCGGCAATC	TAAAAATTGCGGGCCGTCTA	55.0	45.0	58.1	57.9	2229-2257 bp	1.0	79.4
rpl16_p2	CTCTCAGTTCCTCGGCAATC	CTTCTCATTCTGGCCAACGA	55.0	50.0	58.1	57.9	2046 bp	0.166667	45.3
rpl16_p3	CTCTCAGTTCCTCGGCAATC	TCTTCTCATTCTGGCCAACG	55.0	50.0	58.1	57.9	2047 bp	0.166667	45.3
rpl16_p4	TCAGCAATAGTGTCCTTGCC	CTTCTCATTCTGGCCAACGA	50.0	50.0	57.9	57.9	2255 bp	0.166667	45.0
rpl16_p5	TCAGCAATAGTGTCCTTGCC	TCTTCTCATTCTGGCCAACG	50.0	50.0	57.9	57.9	2256 bp	0.166667	45.0