Hotspot

ndhA

SSC · 111602-113708 · 2107 bp

Back to Zoysia

Pi 0.000633
MarkerSeek score 45.50
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2107
variable_sites
4
pi
0.000633
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 ACCCAGTTATCATAGGGAACA ATGGGGGAAGTTGATCGTTG 42.9 50.0 55.7 57.9 2244 bp 1.0 53.0
ndhA_p2 ACCCAGTTATCATAGGGAACA GGGGGAAGTTGATCGTTGAA 42.9 50.0 55.7 57.8 2242 bp 1.0 52.5
ndhA_p3 GAACCCAGTTATCATAGGGAACA ATGGGGGAAGTTGATCGTTG 43.5 50.0 57.6 57.9 2246 bp 1.0 52.2
ndhA_p4 TGAACCCAGTTATCATAGGGAAC ATGGGGGAAGTTGATCGTTG 43.5 50.0 57.6 57.9 2247 bp 1.0 52.2
ndhA_p5 GAACCCAGTTATCATAGGGAACA GGGGGAAGTTGATCGTTGAA 43.5 50.0 57.6 57.8 2244 bp 1.0 51.6